Yes we do. Our whole genome sequencing services are readily available worldwide.
We offer WGS services using the protocols specified in the table below:
* If libraries fall outside of these parameters, the researcher will be contacted for how they would like to proceed.
To assist in determining which protocol would best suit the needs of your project, please contact us.
DNA should be extracted using Qiagen DNeasy / Tissue Extraction kits or equivalent and submitted in Qiagen Elution Buffer (EB), Illumina Resuspension Buffer (RSB) or similar (10mM Tris / 1mM EDTA). Samples must be pure, intact, of high molecular weight and free of contaminating nucleic acids. Samples cannot be derived from FFPE material, should not be modified prior to shipping (e.g. bisulphite conversion) and must not be amplified. Sample quantification must be completed using a fluorometric assay specific to dsDNA (e.g. Picogreen, Qubit BR assay) and meet the following specifications:
Researchers are given access to an online electronic manifest into which several sample characteristics, required for the processing of the sample, must be entered. The manifest is pre-filled with a barcode corresponding to each FluidX 2D tube provided for your samples. The barcode provides a unique identification code for each submitted sample.
DNA must be aliquoted into the pre-allocated FluidX 2D barcoded tubes provided by Genome.One. Samples sent in any other container will not be processed. Please ensure the sample details entered for each barcode in the manifest, match the sample aliquoted into the corresponding barcoded tube. Please ensure that the barcoded tubes sit securely in the rack (if provided) and the lid is locked using the orange clips on the side of the rack. Please ensure the rack containing the barcoded tubes are packaged into an appropriately rigid shipping container.
If no rack is provided, prevent damage to your tubes in transit by ensuring the barcoded tubes are stored securely as follows:
- place the Primary Receptacle (FluidX 2D tube) into a Secondary Receptacle (tube rack, Falcon tube or BioBottle)
- place the Secondary Receptacle into a Tertiary Receptacle (padded outer packaging)
- package the Tertiary Receptacle into an appropriately rigid shipping container
Please affix the provided shipping label containing the manifest ID to the outside of the shipping container. Samples can be shipped at ambient temperature or on dry ice.
Data will be delivered via DNAnexus, a cloud-based platform. Your data will be shipped with a detailed project summary report describing the yield and quality of sequence data for each sample. With your data, you will also find documentation that describes the file structure of your sequencing data and a project summary report. In accordance with Illumina’s specification for the HiSeq X, we guarantee yield ≥100Gb and Q30 ≥75% of bases.
These data are from the Coriell Cell Repository NA12878 reference cell line, which has been extensively analysed by the Genome in a Bottle Consortium. The sequencing libraries were generated with Illumina’s TruSeq Nano V2.5 kit using the Hamilton Microlab STAR robotics platform, achieving >400bp inserts. Each library was sequenced on a single lane of an Illumina HiSeq X patterned flow cell, achieving over 130Gb of yield, with > 83% of bases with quality > Q30 in just 2.8 days. The four data sets are of similar quality, and are provided to allow you to assess the reproducibility of the technology. Each data set substantially surpasses the minimum coverage and quality guaranteed by Illumina and is indicative of the potential for the Illumina HiSeq X Ten sequencing system.
Each of the four datasets consists of raw paired-end data (fastq.gz files) and results obtained with the GATK DNAseq best practices pipeline run on each library independently with the recommended parameters for whole genome sequencing.
Download the data to your computer or server, using the links below.
VECTIS is an integrated genomics platform to house, query, filter and enable easy analysis of genome data from diverse cohorts of any size. Every whole genome sequenced contains around 5 million variants from the reference genome, is approximately 250 Gb in size, and requires 700 hours of processing on high-end computers. VECTIS was developed to suit the needs of diverse users including scientists, clinicians, bioinformaticians and patients. The VECTIS platform will enable increasingly complex queries to facilitate the discovery of new genotype-phenotype associations and scale to meet the increasing demands of large-scale genomics projects.
Please click here to view the VECTIS platform, which has been custom designed and adapted for the Sydney Genomics Collaborative's Medical Genome Reference Bank.
We have flexible payment options available. Please contact us to discuss further.